![]() hominis and Ureaplasma urea- lyticum/ parvum for a total of 288 specimens Table 1: Sensitivity of culture and PCR for M. Five μl extract was then used for amplification with the AnyplexTM II STI-7 kit (Seegene, Korea) which allows the semi-quantitative detection of 7 uro- genital pathogens (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, M. Nucleic acids were extracted from 200 μl eSwab™ liquid Amies medium using the easyMAGTM extraction system with an elution volume of 50 μl. Results were recorded for 3 days with semi-quantitative infor- mation (negative, +, ++, +++) for the solid medium. 25 μl each) of the inoculated LYO 2 broth and incubated anaerobically at 37oC. Solid Mycoplasma/Ureaplasma Agar (Oxoid, UK) was inoculated with 3 single drops (approx. 100 μl) of eSwab medium and incubated aerobically at 37oC. Liquid Urea-Arginine LYO 2 broth (3 ml bioMérieux, France) was inoculated with 4x drops (approx. Seven specimens were deleted from the analysis due to incomplete re- cording of results (therefore, numbers differ slightly from the abstract) The time between taking the specimens and inoculation was retrieved retrospectively from the laboratory information system where available. to estimate survival of these organisms in eSwab™ collection device.Ī total of 295 urogenital specimens submitted for diagnostic workup using the eSwab™ collection kit were included in the study. determine the relative frequencies of Uu and Up and 3. compare semi-quantitative results of culture and PCR 2. parvum (Up) which may have a different clinical significance. Further, culture methods – in contrast to PCR – usually do not allow discrimination of U. The eSwab™ collection kit, a modified liquid Amies transport medium with a regular shape FLOQSwab ® (COPAN) is widely used for urogenital infections but its suitability for culturing Mycoplasma and Ureaplasma species has never been thoroughly investigated. ![]()
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